Ax R L, Bushmeyer S M, Boehm S K, Bellin M E
Endocr Res. 1984;10(1):63-72. doi: 10.1080/07435808409046766.
Bovine ovarian follicles were individually aspirated to obtain granulosa cells and follicular fluid, and estrogen concentrations in fluid were measured with a radioimmunoassay. Granulosa membranes were harvested by centrifugation and were pooled from follicles containing statistically high or low estrogen. Granulosa cell concentrations were estimated by comparing their protein content to cell standards. Results showed that cell numbers increased with increasing follicle size, and follicles with high estrogen concentrations possessed more granulosa than follicles with low estrogen. Specific binding of [3H]heparin decreased with advancing follicle size and was higher to granulosa harvested from follicles containing low estrogen.
对牛卵巢卵泡进行单独抽吸以获取颗粒细胞和卵泡液，并用放射免疫测定法测量卵泡液中的雌激素浓度。通过离心收集颗粒细胞膜，并从含有统计学上高雌激素或低雌激素的卵泡中汇集。通过将颗粒细胞的蛋白质含量与细胞标准进行比较来估计颗粒细胞浓度。结果表明，细胞数量随卵泡大小增加而增加，雌激素浓度高的卵泡比雌激素浓度低的卵泡拥有更多的颗粒细胞。[3H]肝素的特异性结合随卵泡大小增加而降低，并且对从含有低雌激素的卵泡中收获的颗粒细胞的结合更高。
