Burley R W, Sleigh R W, Shenstone F S
Eur J Biochem. 1984 Jul 2;142(1):171-6. doi: 10.1111/j.1432-1033.1984.tb08266.x.
As part of a study of the transfer of proteins and lipids from avian blood to egg yolk, some properties of lipoproteins from the blood of laying hens were compared with those of the low-density lipoprotein (YLP) from egg yolk of the same hens. Although it is known from previous work that particles of the very-low-density lipoprotein (VLDL) of blood are the most likely precursors of YLP, their apoprotein patterns are different, according to electrophoresis and chromatography, with only one protein in common. YLP has the more complicated pattern which does not, however, include apoprotein B (ApoB) the main apoprotein of VLDL. It is suggested that during the transfer of VLDL to yolk, ApoB is cleaved to give smaller yolk apoproteins, especially apovitellenins IV and VI. Some evidence for this suggestion from the similarity of protein digests is presented.
作为对蛋白质和脂质从禽血向蛋黄转移研究的一部分，将产蛋母鸡血液中脂蛋白的某些特性与同一母鸡蛋黄中的低密度脂蛋白（YLP）的特性进行了比较。尽管从先前的研究工作中已知血液中极低密度脂蛋白（VLDL）颗粒最有可能是YLP的前体，但根据电泳和色谱分析，它们的载脂蛋白模式不同，只有一种蛋白质相同。YLP的模式更为复杂，不过它不包括VLDL的主要载脂蛋白载脂蛋白B（ApoB）。有人提出，在VLDL向蛋黄转移的过程中，ApoB被切割产生较小的蛋黄载脂蛋白，尤其是卵黄磷蛋白IV和VI。文中给出了一些来自蛋白质消化物相似性的支持这一观点的证据。
