Newcomb R, Christie W B, Rowson L E
Vet Rec. 1978 May 13;102(19):414-7. doi: 10.1136/vr.102.19.414.
The non-surgical recovery of bovine embryos was examined using a three-lumen PVC catheter passed to the tip of each uterine horn. The recovery of eggs placed in Dulbecco's phosphate buffered saline, with protein was very efficient whether in boiling tubes or funnels. In the absence of protein egg recovery was considerably lower. In a group of 42 heifers, superovulated on two occasions, embryos were recovered non-surgically on day 7 after the first superovulation (Day 0 = oestrus) and surgically after the second. Laparotomy was performed on each occasion and the number of corpora lutea counted. Ovulation rate was lower at the second superovulation and a correspondingly greater recovery of embryos was obtained surgically (P less than 0.02). Non-surgical recovery rates from anaesthetised cows and heifers and from standing sedated heifers were found to be similar. The distance of the catheter tip from the utero-tubal junction (UTJ) significantly affected recovery rate (P less than 0.001). Best results were obtained when the catheter tip was within 5 cm of the UTJ. Most embryos were recovered in the first 100 ml of recovered medium. The method compares favourably with other reported methods.
使用一根三腔聚氯乙烯导管插入每个子宫角尖端，对牛胚胎的非手术回收进行了研究。无论是置于含蛋白质的杜氏磷酸盐缓冲盐水中的卵子，放在试管还是漏斗中，回收效率都很高。在没有蛋白质的情况下，卵子回收率要低得多。在一组42头经两次超排的小母牛中，在第一次超排后第7天（第0天=发情期）进行非手术回收胚胎，第二次超排后进行手术回收。每次都进行剖腹手术并统计黄体数量。第二次超排时排卵率较低，相应地通过手术回收的胚胎数量更多（P<0.02）。发现麻醉的母牛和小母牛以及站立镇静的小母牛的非手术回收率相似。导管尖端距子宫输卵管交界处（UTJ）的距离显著影响回收率（P<0.001）。当导管尖端距UTJ在5厘米以内时，可获得最佳结果。大多数胚胎是在回收的最初100毫升培养基中回收的。该方法与其他报道的方法相比具有优势。
