针对人绒毛膜促性腺激素的单克隆抗体的筛选，该抗体定位于生长在叙利亚仓鼠颊囊内的人绒毛膜癌中。

Selection of monoclonal antibody to hCG which localizes in human choriocarcinoma growing in the Syrian hamster cheek pouch.

作者信息

Khazaeli M B, Brown L E, Kabza G A, Sargent E W, Beierwaltes W H

出版信息

Hybridoma. 1984;3(1):41-8. doi: 10.1089/hyb.1984.3.41.

DOI:10.1089/hyb.1984.3.41

Abstract

Six different monoclonal antibodies (MAbs) to human chorionic gonadotropin (hCG) were evaluated for radioimmunodetection in Golden Syrian Hamsters bearing a human choriocarcinoma in the cheek pouch (188-516 mIU of hCG/g). The affinity of three antibodies when determined were high and five of the antibodies were of IgG1 subclass. After i.v. injection of Staphylococcus Protein A (SPA) affinity purified radioiodinated antibodies, tissue distribution studies demonstrated that antibody 061 had higher target-to-nontarget ratios than the other five antibodies. The mean tumor:muscle ratio of 131I-MAb 061 was 27.5 in four animals, where the mean tumor:muscle ratio of four animals injected with 131I-labeled control monoclonal IgG1 Kappa from MOPC-21 murine myeloma (MOPC-21) was only 2.2. Sharp images of the cheek pouch tumor were obtained at five days following a tracer dose of 131I-061 MAb, but not with the same dose of a 131I-MOPC-21.

摘要

对六种不同的抗人绒毛膜促性腺激素（hCG）单克隆抗体（MAb）进行了评估，以用于在颊囊接种人绒毛膜癌的金黄叙利亚仓鼠（hCG含量为188 - 516 mIU/g）中进行放射免疫检测。所测定的三种抗体的亲和力较高，且其中五种抗体属于IgG1亚类。静脉注射葡萄球菌蛋白A（SPA）亲和纯化的放射性碘化抗体后，组织分布研究表明，抗体061的靶标与非靶标比率高于其他五种抗体。在四只动物中，131I - MAb 061的平均肿瘤:肌肉比率为27.5，而在四只注射了来自MOPC - 21鼠骨髓瘤（MOPC - 21）的131I标记的对照单克隆IgG1 κ链的动物中，平均肿瘤:肌肉比率仅为2.2。在注射示踪剂量的131I - 061 MAb五天后，获得了颊囊肿瘤的清晰图像，但相同剂量的131I - MOPC - 21则未获得。