Zimmermann I, Stern J, Frank F, Keiditsch E, Hofstetter A
Lasers Surg Med. 1984;4(2):167-72. doi: 10.1002/lsm.1900040208.
Lymphatic drainage can be demonstrated by colloidal indian ink injection. A small intramural indian ink spot in the bladder dome is separated from the rest of the rat urinary bladder by encircling transmural Neodymium-YAG (Nd:YAG) laser irradiation. In untreated animals there is an ink flow via the lymphatic vessels to the regional lymph nodes within several hours. In treated animals, after laser irradiation, the indian ink remains in the bladder dome and a penetration of the irradiated area cannot be found by macroscopic and histologic examination. Indian ink will be found in the regional lymph nodes after the ninth day of absorption of the coagulation necrosis and recanalisation of the intercepted lymphatics. Evidence for the interception of the lymphatic drainage is demonstrated by the distinctly delayed ink flow and the injected ink's remaining in the irradiated area. From this, we conclude that Nd:YAG laser irradiation is especially suitable for tumor coagulation, since simultaneous interception of lymphatic drainage will inhibit the spreading of tumor cells.
淋巴引流可通过注射胶体印度墨汁来显示。在膀胱顶部的膀胱壁内注射一个小的印度墨汁点，并用环形透壁钕钇铝石榴石（Nd:YAG）激光照射将其与大鼠膀胱的其余部分隔开。在未处理的动物中，数小时内墨汁会通过淋巴管流向局部淋巴结。在经过激光照射的处理动物中，印度墨汁会留在膀胱顶部，通过肉眼和组织学检查均未发现照射区域有墨汁渗透。在凝固性坏死吸收和被阻断的淋巴管再通后的第九天，可在局部淋巴结中发现印度墨汁。墨汁流动明显延迟以及注入的墨汁留在照射区域，证明了淋巴引流被阻断。由此我们得出结论，Nd:YAG激光照射特别适用于肿瘤凝固，因为同时阻断淋巴引流将抑制肿瘤细胞的扩散。
