Chakravarty B, Mills I H, Callingham B A
Ren Physiol. 1984;7(4):205-17. doi: 10.1159/000172940.
Freeze-dried samples of human urine were reconstituted and chromatographed on Sephadex G-25 columns. Fractions possessing natriuretic activity were eluted after the salts. Application of aliquots of these fractions, after further freeze drying and reconstitution, to the isolated anococcygeus muscle of the rat produced dose-dependent contractions of this smooth muscle. The potency of these samples in causing contractions was positively correlated with their ability to cause natriuresis in water-loaded conscious rats. Use of selective antagonists of possible agonistic agents showed that the ability of the samples to contract smooth muscle was not due to catecholamines, acetylcholine, 5-hydroxytryptamine, prostaglandin E and angiotensin II.
将人尿液的冻干样本复溶，并在葡聚糖G - 25柱上进行色谱分析。具有利钠活性的组分在盐分之后被洗脱。将这些组分的等分试样进一步冻干并复溶后，应用于大鼠分离的尾骨肌，可引起该平滑肌剂量依赖性收缩。这些样本引起收缩的效力与其在水负荷清醒大鼠中引起利钠作用的能力呈正相关。使用可能的激动剂的选择性拮抗剂表明，样本收缩平滑肌的能力并非由于儿茶酚胺、乙酰胆碱、5 - 羟色胺、前列腺素E和血管紧张素II。
