Senior P V, Sunter J P, Appleton D R, Watson A J
J Anat. 1984 Oct;139 ( Pt 3)(Pt 3):513-23.
At eight time points over a period of 24 hours, crypt length (in cells) and crypt cell birth rate were measured by the stathmokinetic method with vincristine and evaluated at two sites in the small intestine and two in the colon in Wistar Porton rats. Normal animals were compared with animals which had received 24 injections, at weekly intervals, of the intestinal carcinogen 1,2-dimethylhydrazine. In the treated animals, crypts were significantly longer at sites prone to tumour formation but not at those sites known to be resistant to the effects of the carcinogen. In neither group of animals was there any significant fluctuation in mean crypt length over a 24 hours period. Crypt cell birth rates showed a considerable fluctuation. No difference was noted between normal animals and those treated with dimethylhydrazine and statistical analysis failed to confirm the presence of any true periodic fluctuation.
在24小时内的八个时间点，采用长春新碱的静止动力学方法测量Wistar Porton大鼠小肠两个部位和结肠两个部位的隐窝长度（以细胞数计）及隐窝细胞增殖率。将正常动物与每周接受24次肠道致癌物1,2 - 二甲基肼注射的动物进行比较。在经处理的动物中，易于形成肿瘤的部位隐窝明显更长，但对致癌物作用有抗性的部位则不然。两组动物在24小时内平均隐窝长度均无显著波动。隐窝细胞增殖率有相当大的波动。正常动物与用二甲基肼处理的动物之间未发现差异，统计分析未能证实存在任何真正的周期性波动。
