Senior P V, Sunter J P, Appleton D R, Watson A J
Br J Cancer. 1984 Mar;49(3):281-90. doi: 10.1038/bjc.1984.45.
Mucosal explants were prepared from the colons of normal rats and from the non-neoplastic colonic mucosa of rats which had been treated chronically with the intestinal carcinogen dimethylhydrazine. They were maintained in an organ culture system which permitted survival up to at least 25 days. Morphological preservation of the mucosa was excellent up to 6 days in culture and thereafter changes began to occur. But even at 25 days normal crypt structures were still evident. The hyperplastic and dysplastic changes seen in pre-culture samples of DMH-treated mucosae remained recognisable during the first two days in culture. They were no longer seen in explants examined after this time however and, indeed, there appeared to be no difference in the morphology and survival of control and DMH-treated mucosae. It is possible that our culture system does not permit further neoplastic progression, but an alternative explanation is that the system discriminates specifically against the survival of neoplastic elements.
黏膜外植体取自正常大鼠的结肠以及长期用肠道致癌物二甲基肼处理的大鼠的非肿瘤性结肠黏膜。将它们置于器官培养系统中，该系统可使外植体存活至少25天。培养6天内黏膜的形态保存良好，此后开始出现变化。但即使在25天时，正常隐窝结构仍很明显。在培养的前两天，二甲基肼处理的黏膜培养前样本中所见的增生和发育异常变化仍可辨认。然而，在此之后检查的外植体中不再能看到这些变化，实际上，对照和二甲基肼处理的黏膜在形态和存活方面似乎没有差异。有可能我们的培养系统不允许进一步的肿瘤进展，但另一种解释是该系统特别不利于肿瘤成分的存活。
