McGrath J, Solter D
Science. 1984 Dec 14;226(4680):1317-9. doi: 10.1126/science.6542249.
More than 90 percent of enucleated one-cell mouse embryos receiving pronuclei from other one-cell embryos successfully develop to the blastocyst stage in vitro. In this investigation, nuclei from successive preimplantation cleavage stages were introduced into enucleated one-cell embryos and the embryos were tested for development in vitro. Although two-cell nuclei supported development to the morula or blastocyst stage, four-cell, eight-cell, and inner cell mass cell nuclei did not. The inability of cell nuclei from these stages to support development reflects rapid loss of totipotency of the transferred nucleus and is not the result of simultaneous transfer of membrane or cytoplasm.
超过90%接受来自其他单细胞胚胎原核的去核单细胞小鼠胚胎在体外成功发育到囊胚阶段。在本研究中，将植入前连续分裂阶段的细胞核导入去核的单细胞胚胎，并对这些胚胎进行体外发育测试。虽然二细胞期细胞核支持胚胎发育到桑椹胚或囊胚阶段，但四细胞期、八细胞期和内细胞团细胞核却不能。这些阶段的细胞核无法支持发育反映了转移细胞核全能性的快速丧失，而不是同时转移细胞膜或细胞质的结果。
