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饲养管理、寄生虫及其他疾病作为影响猪旋毛虫病酶联免疫吸附测定(ELISA)可靠性的因素

Husbandry, parasitic and other diseases as factors in the reliability of the enzyme linked immunosorbent assay (ELISA) for detection of trichinellosis in pigs.

作者信息

van Knapen F, Franchimont J H, Skovgaard N, Guil'dal J, Henriksen S A

出版信息

Vet Parasitol. 1984 Oct;16(1-2):17-22. doi: 10.1016/0304-4017(84)90004-9.

Abstract

Serum samples were obtained from pigs originating from specified pathogen-free farms, large industrialised farms and small conventional farms. All animals proved to be free of Trichinella spiralis by a pooled sample digestion method. Careful meat inspection studies on parasitic infections other than trichinellosis, and other inflammatory reactions were recorded and used for subdivision of the animals in different groups. It was concluded that animal husbandry, parasitic infections, or inflammatory reactions have not influenced the mean extinction values of an ELISA for the detection of T. spiralis antibodies, however, the specified pathogen free animals yielded somewhat lower results.

摘要

血清样本取自来自特定无病原体农场、大型工业化农场和小型传统农场的猪。通过混合样本消化法证明所有动物均未感染旋毛虫。记录了除旋毛虫病以外的寄生虫感染以及其他炎症反应的仔细肉类检查研究结果,并用于将动物分为不同组。得出的结论是,畜牧业、寄生虫感染或炎症反应并未影响用于检测旋毛虫抗体的ELISA的平均消光值,然而,特定无病原体的动物所得结果略低。

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