采用酶联免疫吸附测定法和酶联免疫电转移印迹法对各种疾病患者针对旋毛虫抗原的交叉反应进行研究。
Investigation of cross-reactions against Trichinella spiralis antigens by enzyme-linked immunosorbent assay and enzyme-linked immunoelectrotransfer blot assay in patients with various diseases.
作者信息
De-la-Rosa J L, Alcantara P, Correa D
机构信息
Departamento de Inmunoparasitología, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, Secretaria de Salud, México D.F., México.
出版信息
Clin Diagn Lab Immunol. 1995 Jan;2(1):122-4. doi: 10.1128/cdli.2.1.122-124.1995.
Abstract
Data regarding cross-reactions against Trichinella spiralis in humans are scarce and controversial. For this reason, we tested serum samples from patients with typhoid fever, brucellosis, toxoplasmosis, amoebiasis, cysticercosis, trichocephaliasis, ascariasis, and onchocerciasis against an antigenic extract of T. spiralis infective larvae in an enzyme-linked immunosorbent assay (ELISA) and an enzyme-linked immunoelectrotransfer blot (EITB) assay. All except one serum sample from the group of patients with onchocerciasis were negative in the ELISA; in the EITB assay, only faint bands were observed with the samples from patients with onchocerciasis and ascariasis and negative results were obtained with the samples from patients with other diseases. In conclusion, cross-reactions were found only in the groups of patients with other nematode infections and were of very low magnitude, most of them virtually negative.
摘要
关于人类对旋毛虫交叉反应的数据稀缺且存在争议。因此，我们采用酶联免疫吸附测定（ELISA）和酶联免疫电转移印迹（EITB）测定，用旋毛虫感染性幼虫的抗原提取物检测伤寒、布鲁氏菌病、弓形虫病、阿米巴病、囊尾蚴病、毛首鞭形线虫病、蛔虫病和盘尾丝虫病患者的血清样本。除盘尾丝虫病患者组的一份血清样本外，ELISA检测中所有其他样本均为阴性；在EITB测定中，仅在盘尾丝虫病和蛔虫病患者的样本中观察到微弱条带，其他疾病患者的样本检测结果均为阴性。总之，仅在其他线虫感染患者组中发现交叉反应，且反应程度非常低，大多数几乎呈阴性。
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