Vitkov M
Vet Med Nauki. 1984;21(9):52-6.
The passive hemagglutination test was employed to establish the presence of protein A in a total of 133 strains of coagulase-positive and coagulase-negative staphylococci isolated from sheep with mastitis. Defined were also the antigen structure, phage behaviour, and some biologic properties of these staphylococci. It was found that 91 per cent out of 110 strains of Staphylococcus aureus studied produced protein A. At the same time not one of the 23 strains of Staphylococcus epidermidis showed the presence of this surface antigen. This data showed the necessity of introducing the hemagglutination test in routine microbiologic work for the demonstration of protein A in staphylococci isolated in the cases of pathologic processes in animals.
采用被动血凝试验检测从患乳腺炎绵羊分离出的133株凝固酶阳性和凝固酶阴性葡萄球菌中蛋白A的存在情况。同时还确定了这些葡萄球菌的抗原结构、噬菌体行为及一些生物学特性。结果发现,在所研究的110株金黄色葡萄球菌中,91%产生蛋白A。而23株表皮葡萄球菌中无一株显示存在这种表面抗原。这些数据表明,在常规微生物学工作中引入血凝试验以检测动物病理过程中分离出的葡萄球菌中的蛋白A是必要的。