Márquez M T, Ponce-Hornos J E, Aramendía P
Methods Find Exp Clin Pharmacol. 1984 Dec;6(12):755-61.
In the rat left atrium, electrically driven, a superfusion dropping method was developed. The device essentially consisted of a pair of platinum wire electrodes which held one end of the tissue, while the other end was attached to an isometric tension transducer. Either normal or isotopically labeled solutions were poured on the preparation at preset flow rates by means of a minipump. Effluent was collected fractionally in vials for measurement of radioactivity. Several perfusion flows tested led us to the conclusion that higher rates than 2.26 ml/min did not improve contractile performance. Stability of the preparation was found similar to the classical isolated organ-bath method during a 180 min period of observation. Set-up of the superfused atria reproduced paired determinations of 45Ca efflux, together with the contractile parameters. In addition, the frequency of sampling (intervals of 1 min or less) allowed us to detect changes in the washout pattern of exchangeable 45Ca even during early phases of Ca efflux. Therefore, the method reported herein seems to overcome the serious handicap of the scanty tissue employed.
在大鼠左心房中，采用电驱动的方式开发了一种灌流滴注法。该装置主要由一对铂丝电极组成，电极夹住组织的一端，而另一端连接到等长张力换能器上。通过微型泵以预设流速将正常溶液或同位素标记溶液灌注到标本上。流出液分份收集到小瓶中用于测量放射性。对几种灌注流速进行测试后，我们得出结论，流速高于2.26 ml/min并不能改善收缩性能。在180分钟的观察期内，发现该标本的稳定性与经典的离体器官浴法相似。灌流心房的设置重现了对45Ca流出量的配对测定以及收缩参数。此外，采样频率（间隔1分钟或更短）使我们即使在Ca流出的早期阶段也能检测到可交换45Ca洗脱模式的变化。因此，本文报道的方法似乎克服了所用组织量少这一严重缺陷。
