Schlegel W, Grube D
Z Gastroenterol. 1978 May;16(5):304-10.
A further specific, reproducible radioimmunoassay of cholecystokinin-pancreozymin was developed. 125J-labelled 39-CCK was used as tracer. The limit of detection for CCK in serum was 40 pg/ml. Basal levels for CCK have been found to be 222 pg/ml, being increased after food ingestin. No crossreactivity with gastronintestinal hormones could be observed. Somatostatin was inhibiting the stimulated CCK-release. Histochemical and radioimmunological determinations localized CCK-containing cells in duodenal tissue of humans. Physiological and pathological processes in which CCK is involved have to be studied to evaluate its role in the gastrointestinal tract.
开发了一种更特异、可重复的胆囊收缩素-促胰酶素放射免疫测定法。使用125J标记的39-CCK作为示踪剂。血清中CCK的检测限为40 pg/ml。已发现CCK的基础水平为222 pg/ml，进食后会升高。未观察到与胃肠激素的交叉反应。生长抑素可抑制CCK的刺激释放。组织化学和放射免疫测定法将含CCK的细胞定位在人类十二指肠组织中。必须研究CCK参与的生理和病理过程，以评估其在胃肠道中的作用。
