Locker R H, Wild D J
J Ultrastruct Res. 1984 Sep;88(3):207-22. doi: 10.1016/s0022-5320(84)90119-9.
The N-lines of skeletal muscle, although known for a century, have remained elusive and neglected structures. Their fortuitous appearance in diverse experiments on beef sternomandibularis muscle, involving stretch, heat denaturation, and myosin extraction, have been collected and related to the literature. Treatments with pyroantimonate and formamide solutions have proved a more reliable way of visualizing N-lines. It is concluded that there are at least seven N-lines: an N1-line always near the Z-line, four N2-lines in the mid-I-band, and two N3-lines at the extremity of the I-filaments or in the "gap." There is evidence for suspension of both N2- and N3-lines on G-filaments.
骨骼肌的N线，尽管已被知晓一个世纪，但仍然是难以捉摸且被忽视的结构。它们在对牛胸骨下颌肌进行的涉及拉伸、热变性和肌球蛋白提取的各种实验中的偶然出现情况已被收集并与文献相关联。用焦锑酸盐和甲酰胺溶液处理已被证明是一种更可靠的观察N线的方法。得出的结论是，至少有七条N线：一条N1线总是靠近Z线，四条N2线在I带中部，两条N3线在I细丝末端或“间隙”中。有证据表明N2线和N3线都悬浮在G细丝上。
