Leadem C A, Blask D E
J Pineal Res. 1984;1(3):263-72. doi: 10.1111/j.1600-079x.1984.tb00217.x.
The purpose of this study was to define the peripubertal changes in prolactin (PRL) synthesis in blind-anosmic female rats. To do this prepubertal female rats were either rendered blind-anosmic (BA) or blind-anosmic-pinealectomized (BAP) or left intact (INT). Half the animals were killed after 1 wk (before puberty) while the remaining animals were terminated after 4 wk (after puberty). Pituitary PRL synthesis was measured by the in vitro incorporation of 3H-leucine into PRL. Anterior pituitary weight and DNA content did not differ between BA, BAP, and INT animals 1 wk after treatment. There was a 65% increase in weight and a 55% increase in DNA content in INT pituitaries between 1 and 4 wk. While these increases were also present in BAP rats, they were almost entirely prevented in BA animals. PRL synthesis followed a similar pattern with a 78% increase in synthesis in INT groups between 1 and 4 wk which was, once again, almost entirely absent in BA rats. Pinealectomy prevented this effect. Blinding and anosmia in postpubertal animals produced only a minor decrease in PRL synthesis and pituitary weight and no decrease in pituitary cell number. From these data we conclude that the pineal can inhibit the normal proliferation of PRL cells that occurs during puberty in blind-anosmic female rats.
本研究的目的是确定盲嗅缺失雌性大鼠青春期前泌乳素（PRL）合成的变化。为此，将青春期前雌性大鼠分为盲嗅缺失组（BA）、盲嗅缺失-松果体切除组（BAP）或完整组（INT）。一半动物在1周后（青春期前）处死，其余动物在4周后（青春期后）处死。通过体外将3H-亮氨酸掺入PRL来测量垂体PRL的合成。治疗1周后，BA、BAP和INT组动物的垂体前叶重量和DNA含量没有差异。在1至4周期间，INT组垂体重量增加65%，DNA含量增加55%。虽然BAP大鼠也有这些增加，但在BA组动物中几乎完全被阻止。PRL合成遵循类似模式，INT组在1至4周期间合成增加78%，而BA大鼠再次几乎完全没有这种增加。松果体切除可防止这种效应。青春期后动物的失明和嗅觉缺失仅导致PRL合成和垂体重量略有下降，垂体细胞数量没有减少。从这些数据中我们得出结论，松果体可以抑制盲嗅缺失雌性大鼠青春期期间发生的PRL细胞的正常增殖。
