Welsh M G
J Pineal Res. 1984;1(4):305-16. doi: 10.1111/j.1600-079x.1984.tb00221.x.
The potassium pyroantimonate-osmium method was employed to determine the distribution of cations in the superficial pineal gland of the Mongolian gerbil. The reaction product in the glands was presumed to be calcium following the use of both the chelator EGTA and x-ray microanalysis. A gradient of reaction product was found in the pineal with the highest concentrations occurring in and around the pineal concretions (PC). The periphery of mature PC demonstrated a heavy precipitate as did the surrounding pinealocytes. PC-associated pinealocytes contained reaction product in many organelles, including small vesicles that appeared to be opening in the direction of the concretions. The vacuoles of the pinealocytes contained various concentrations of precipitate. The results obtained in the present investigation have led to a hypothesis on the formation of pineal concretions: a pinealocyte vacuole is formed, calcium is transported to the vacuole, the cation content of the vacuole increases, and the cations precipitate into an immature concretion. Fibrillar material and an amorphous substance located within the pinealocyte vacuole appear to form cation containing vesicles that contribute to the periphery of the developing PC, resulting in the continued growth of the concretion.
采用焦锑酸钾锇法测定蒙古沙鼠松果体浅层中阳离子的分布。在用螯合剂乙二醇双四乙酸（EGTA）和X射线微量分析后，腺体中的反应产物被推测为钙。在松果体中发现了反应产物的梯度分布，其最高浓度出现在松果体钙化物（PC）及其周围。成熟PC的周边显示出大量沉淀，周围的松果体细胞也是如此。与PC相关的松果体细胞在许多细胞器中含有反应产物，包括一些似乎朝着钙化物方向开口的小泡。松果体细胞的液泡含有不同浓度的沉淀。本研究获得的结果导致了一个关于松果体钙化物形成的假说：形成一个松果体细胞液泡，钙被转运到液泡中，液泡中的阳离子含量增加，阳离子沉淀形成不成熟的钙化物。位于松果体细胞液泡内的纤维状物质和无定形物质似乎形成了含阳离子的小泡，这些小泡有助于发育中的PC的周边形成，从而导致钙化物的持续生长。
