Perbeck L, Olsson L, Thulin L
Microcirc Endothelium Lymphatics. 1984 Oct;1(5):611-28.
Fluorescein angiography (FA), which implies that the tissue is reproduced in natural size, and fluorescence microscopy was compared in anesthetized normovolemic and hypovolemic rats in order to define the microscopic background to the fluorescence pattern obtained by FA. It was found that the fluorescence detected by FA by the use of sodium fluorescein as an indicator substance was emitted from the interstitial and intracellular compartments, including the intranuclear. Microscopy showed that sodium fluorescein was located extravascularly in the small intestine within 4 seconds after an intravenous injection. There was a 4 second interval between corresponding microscopic and macroscopic fluorescence pattern.
荧光血管造影术（FA）可按自然大小再现组织，为明确FA所获荧光模式的微观背景，对麻醉状态下的正常血容量和低血容量大鼠进行了荧光血管造影术与荧光显微镜检查的比较。结果发现，以荧光素钠作为指示物质，FA检测到的荧光发自间质和细胞内区室，包括细胞核内。显微镜检查显示，静脉注射后4秒内，荧光素钠位于小肠的血管外。相应的微观和宏观荧光模式之间有4秒的间隔。
