Darzynkiewicz Z, Carter S
J Cell Physiol. 1984 Apr;119(1):1-6. doi: 10.1002/jcp.1041190102.
Compound 48/80 (poly-p-methoxyphenethylmethylamine), an agent commonly used to trigger degranulation of mast cells, at concentrations of 5-20 micrograms/ml suppresses the proliferation of L1210 and Friend leukemic cells in vitro, inducing the formation of giant cells, which are polykaryons. Both the proportion of polykaryons in cultures and their size (which reflects the number of nuclei per polykaryon) increase during growth in the presence of 48/80 up to 48 hr; thereafter, the cells lose viability. A predominant number of nuclei in these polykaryons contain a 4C, or higher DNA content. The data indicate that compound 48/80 impairs the cleavage (cytokinesis) and perhaps mitotic processes. Mechanisms by which compound 48/80 induces the described effects are unknown but may be related to the polycationic nature of the polymer and its interaction with the cell membrane. Certain attributes of compound 48/80 suggest that this or similar polymers may have value as research tools for the study of regulatory mechanisms involved in cell division.
化合物48/80（聚对甲氧基苯乙甲胺）是一种常用于触发肥大细胞脱颗粒的试剂，在浓度为5 - 20微克/毫升时，可在体外抑制L1210和弗氏白血病细胞的增殖，诱导形成多核巨细胞。在48/80存在的情况下培养长达48小时期间，培养物中多核巨细胞的比例及其大小（反映每个多核巨细胞核的数量）均会增加；此后，细胞失去活力。这些多核巨细胞中的大多数细胞核含有4C或更高的DNA含量。数据表明化合物48/80损害了细胞分裂（胞质分裂），或许还有有丝分裂过程。化合物48/80诱导上述效应的机制尚不清楚，但可能与其聚合物的聚阳离子性质及其与细胞膜的相互作用有关。化合物48/80的某些特性表明，这种或类似的聚合物可能作为研究细胞分裂调控机制的研究工具具有价值。
