Biochemical and immunological characteristics of ferritin from HL-60, U-937 and K-562 cell lines: implications for haemopoietic regulation.

It has been suggested that acidic isoferritins play a major role in the regulation of human granulocyte-macrophage (CFU-GM) proliferation. Such regulatory isoferritins are said to occur in some human tissues including both leukaemic and normal leucocytes, and certain established cell lines. They are glycosylated and bind to Con A. Extracts from HL-60, U-937 and K-562 cell lines are said to contain inhibitory activity but only in HL60 cells is the activity neutralized by antibody to acidic isoferritins. The properties of ferritin from these cell lines have been investigated. HL60 and U-937 extracts contained predominantly basic (spleen-type) isoferritins and K-562 extracts predominantly acidic (heart-type) isoferritins on immunoradiometric assay. None of the ferritin bound to Con A. On anion exchange chromatography ferritin from U-937 was basic in character, that from K-562 was acidic and HL-60 contained a variety of isoferritins. The identification of glycosylated acidic isoferritins as inhibitors of granulopoiesis appears to be an oversimplification and the classification of isoferritins on the basis of two subunit types may also need revision.