1,25 - 二羟基维生素D3对成骨细胞克隆MC3T3 - E1中碱性磷酸酶活性和胶原蛋白合成的影响。

Effect of 1,25-dihydroxyvitamin D3 on alkaline phosphatase activity and collagen synthesis in osteoblastic cells, clone MC3T3-E1.

作者信息

Kurihara N, Ikeda K, Hakeda Y, Tsunoi M, Maeda N, Kumegawa M

出版信息

Biochem Biophys Res Commun. 1984 Mar 15;119(2):767-71. doi: 10.1016/s0006-291x(84)80316-2.

Abstract

A stimulative effect of 1,25-dihydroxyvitamin D3 was tested on osteoblastic cells, clone MC3T3-E1, cultured in serum-free medium with 0.1% bovine serum albumin. This steroid increased alkaline phosphatase activity in a dose-related fashion. The steroid also stimulated dose-dependently collagen and non-collagen protein syntheses, their maximal effects being observed at 12 and 24 h, respectively. The incorporation of [3H]-proline into collagen or non-collagen protein in cells exposed to this steroid for 12 h was 2.9 or 1.9-fold over that of control cultures, respectively. These results strongly indicate the stimulative effects of 1,25-dihydroxyvitamin D3 on the differentiation of osteoblasts in vitro.

摘要

在含有0.1%牛血清白蛋白的无血清培养基中培养的成骨细胞克隆MC3T3-E1上测试了1,25-二羟基维生素D3的刺激作用。这种类固醇以剂量相关的方式增加碱性磷酸酶活性。该类固醇还以剂量依赖性方式刺激胶原蛋白和非胶原蛋白的合成，其最大作用分别在12小时和24小时观察到。在暴露于该类固醇12小时的细胞中，[3H]-脯氨酸掺入胶原蛋白或非胶原蛋白的量分别是对照培养物的2.9倍或1.9倍。这些结果有力地表明了1,25-二羟基维生素D3在体外对成骨细胞分化的刺激作用。