去卵巢大鼠的骨缺损。骨髓基质细胞群的功能作用及口服双氢速甾醇治疗的效果。
Bone deficit in ovariectomized rats. Functional contribution of the marrow stromal cell population and the effect of oral dihydrotachysterol treatment.
作者信息
Tabuchi C, Simmons D J, Fausto A, Russell J E, Binderman I, Avioli L V
出版信息
J Clin Invest. 1986 Sep;78(3):637-42. doi: 10.1172/JCI112621.
Abstract
This study investigates the proliferative and osteogenic role of marrow stromal/osteoprogenitor cells in the development of the cortical bone deficit in ovariectomized (OVX) female rats. In vitro, clonal growth of marrow stromal cells from OVX rats was significantly impaired (vs. sham-operated controls). Yet in vivo, cells from sham-operated and OVX rats had equal osteogenic potential in several in vivo experimental situations, such as in intraperitoneally implanted millipore diffusion chambers and in intramuscular implants of marrow plus osteoinductive bone matrix (composite grafts). Long-term (6 mo) dihydrotachysterol (DHT) treatment of OVX rats enhanced their in vitro proliferative potential and clonal growth, as well as their osteogenic expression in composite grafts. The observation that the in vivo osteogenic performance of OVX rat marrow stromal cells was normal at extraosseous sites suggests that the mechanisms leading to osteopenia may involve an abnormality in cell-matrix interactions.
摘要
本研究调查了骨髓基质/骨祖细胞在去卵巢（OVX）雌性大鼠皮质骨缺损形成过程中的增殖和成骨作用。在体外，OVX大鼠骨髓基质细胞的克隆生长显著受损（与假手术对照组相比）。然而在体内，在几种体内实验情况下，如在腹腔内植入的微孔扩散室以及骨髓加骨诱导性骨基质的肌肉内植入物（复合移植物）中，假手术大鼠和OVX大鼠的细胞具有同等的成骨潜能。对OVX大鼠进行长期（6个月）双氢速甾醇（DHT）治疗可增强其体外增殖潜能和克隆生长，以及其在复合移植物中的成骨表达。OVX大鼠骨髓基质细胞在骨外部位的体内成骨性能正常这一观察结果表明，导致骨质减少的机制可能涉及细胞与基质相互作用的异常。
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