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Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA.

作者信息

Matteson K J, Chung B C, Miller W L

出版信息

Biochem Biophys Res Commun. 1984 Apr 16;120(1):264-70. doi: 10.1016/0006-291x(84)91443-8.

DOI:10.1016/0006-291x(84)91443-8
PMID:6546877
Abstract

P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC X dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.

摘要

相似文献

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Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA.
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2
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引用本文的文献

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Identification and characterization of cDNA clones specific for cholesterol side-chain cleavage cytochrome P-450.胆固醇侧链裂解细胞色素P-450特异性cDNA克隆的鉴定与特性分析
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Molecular loci for potential drug toxicity in ovaries.卵巢中潜在药物毒性的分子位点。
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3
Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta.
人胆固醇侧链裂解酶，P450scc：cDNA克隆、基因定位于15号染色体以及在胎盘中的表达。
Proc Natl Acad Sci U S A. 1986 Dec;83(23):8962-6. doi: 10.1073/pnas.83.23.8962.