Ishiguro T, Nakanishi M
J Biochem. 1984 Feb;95(2):581-3. doi: 10.1093/oxfordjournals.jbchem.a134642.
A new method has been developed for preparation of supported planar lipid membranes containing transmembrane proteins. Phosphatidylcholine and glycophorin A were deposited on the surface of alkylated glass coverslips by dialysis. The planar lipid membranes prepared in this manner were examined for the binding of wheat germ agglutinin agarose. The binding of the agarose to these membranes is dependent on the membrane fluidity. The advantages of this technique are: (1) a supported planar membrane can be more readily prepared, and (2) less amounts of membrane proteins are needed for reconstitution.
一种制备含有跨膜蛋白的支撑平面脂质膜的新方法已经被开发出来。通过透析将磷脂酰胆碱和血型糖蛋白A沉积在烷基化玻璃盖玻片表面。对以这种方式制备的平面脂质膜进行了麦胚凝集素琼脂糖结合检测。琼脂糖与这些膜的结合取决于膜的流动性。该技术的优点是：（1）更容易制备支撑平面膜，（2）重组所需的膜蛋白量更少。
