Nakanishi M
FEBS Lett. 1984 Oct 29;176(2):385-8. doi: 10.1016/0014-5793(84)81202-8.
A procedure for reconstituting a transmembrane protein by the freeze-thaw method into supported planar lipid layers has been developed. A solution containing human glycophorin A was introduced between an alkylated cover glass with lipid layers from soybean phospholipids and a bare glass slide, and was then put in a glass dish which was frozen outside by liquid nitrogen. The lipid layer membranes prepared in this manner have been examined by the binding of both macrophages and wheat germ agglutinin agarose. Macrophages bound more efficiently to the membranes bearing glycophorin A and spread more rapidly than those of the control membranes.
已开发出一种通过冻融法将跨膜蛋白重组到支持的平面脂质层中的方法。将含有人类血型糖蛋白A的溶液引入到带有大豆磷脂脂质层的烷基化盖玻片和裸载玻片之间，然后放入通过液氮在外部冷冻的玻璃培养皿中。通过巨噬细胞和麦胚凝集素琼脂糖的结合对以这种方式制备的脂质层膜进行了检测。与对照膜相比，巨噬细胞更有效地结合到带有血型糖蛋白A的膜上，并且铺展得更快。
