Tway P C, Downing G V, Slayback J R, Rahn G S, Isensee R K
Biomed Mass Spectrom. 1984 Apr;11(4):172-6. doi: 10.1002/bms.1200110407.
A method based on direct exposure, positive ion, chemical ionization mass spectrometry/mass spectrometry (ms/ms) was developed for the confirmatory assay of the antiparasitic drug, ivermectin, in animal tissue. Following extraction, column and preparative liquid chromatography, mass spectrometric/mass spectrometric analysis of the drug in liver samples provided reliable detection limits to 8-10 parts-per-billion at a signal: noise of greater than 10:1. Blank tissue consistently displayed no chemical/matrix interference. Besides the development of a confirmatory assay, the study also demonstrates the analytical capability and the role of MS/MS vis-a-vis other applied mass spectrometric techniques.
开发了一种基于直接暴露、正离子、化学电离质谱/质谱（ms/ms）的方法，用于动物组织中抗寄生虫药物伊维菌素的确证分析。提取、柱色谱和制备液相色谱后，对肝脏样品中的药物进行质谱/质谱分析，在信号噪声大于10:1时，提供了可靠的检测限，低至十亿分之八至十。空白组织始终未显示化学/基质干扰。除了确证分析方法的开发外，该研究还展示了质谱/质谱相对于其他应用质谱技术的分析能力和作用。
