采用带电子捕获检测的气液色谱法测定5-氨基乙酰丙酸合酶活性。
Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.
作者信息
Gorchein A
机构信息
Department of Clinical Pharmacology, St. Mary's Hospital Medical School, London, U.K.
出版信息
Biochem J. 1990 Aug 15;270(1):103-7. doi: 10.1042/bj2700103.
Abstract
A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
摘要
本文描述了一种5-氨基乙酰丙酸合酶活性的特异性测定方法，其灵敏度与放射化学测定法相当。该方法基于气相色谱法，通过电子捕获检测5-氨基乙酰丙酸的乙酰乙酸乙酯吡咯衍生物的五氟苄酯以及用作内标的6-氨基-5-氧代己酸的相应化合物。已在大鼠肝脏、脾脏、肾脏和大脑的匀浆以及人淋巴细胞中测定了酶活性。
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Comparisons and modifications of the colorimetric assay for delta-aminolevulinic acid synthase.δ-氨基乙酰丙酸合酶比色测定法的比较与改进
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