Van Dael H, Ceuterickx P
Chem Phys Lipids. 1984 Jul;35(2):171-81. doi: 10.1016/0009-3084(84)90023-9.
The depression of the phase-transition temperature of dimyristoyl- and dipalmitoylphosphatidylcholine vesicles induced by phenol has been investigated by fluorescence polarization. This effect is strongly pH and concentration dependent. Only the uncharged phenol molecule influences the fluidity of the bilayer so that the interaction of phenol with the bilayer can be situated in the hydrophobic acyl chain region. Direct measurements of the partitioning of phenol in the phospholipid vesicles confirm these results and show a limited and concentration-dependent solubility. Phase-transition temperature depressions, obtained from thermodynamic analysis of partition coefficient measurement, are in good agreement with the experimental values.
通过荧光偏振研究了苯酚诱导的二肉豆蔻酰磷脂酰胆碱和二棕榈酰磷脂酰胆碱囊泡相变温度的降低。这种效应强烈依赖于pH值和浓度。只有不带电荷的苯酚分子会影响双层膜的流动性，因此苯酚与双层膜的相互作用可能发生在疏水酰基链区域。对磷脂囊泡中苯酚分配的直接测量证实了这些结果，并显示出有限的且依赖于浓度的溶解度。从分配系数测量的热力学分析得出的相变温度降低与实验值吻合良好。
