Cook J E, Rankin E C
Neurosci Lett. 1984 Jul 13;48(1):61-6. doi: 10.1016/0304-3940(84)90289-1.
The lectin wheat-germ agglutinin (WGA), conjugated to horseradish peroxidase (HRP), is an effective tracer for goldfish optic axons. Under suitable conditions, WGA-HRP injected iontophoretically into the normal goldfish tectum is taken up by retinotopic optic terminals (but not by axons of passage) and labels a small, discoid patch of retinal ganglion cells. Under identical conditions, unconjugated HRP is mainly taken up by injured axons of passage. WGA-HRP injected into the tectum after regeneration of the contralateral optic nerve again labels a small patch of ganglion cells, demonstrating the extent to which regenerated terminals are also retinotopic.
与辣根过氧化物酶（HRP）结合的凝集素小麦胚凝集素（WGA）是金鱼视神经轴突的一种有效示踪剂。在合适的条件下，通过离子电渗法注入正常金鱼视顶盖的WGA-HRP被视网膜拓扑视神经终末摄取（但不被过路轴突摄取），并标记一小片盘状视网膜神经节细胞。在相同条件下，未结合的HRP主要被受损的过路轴突摄取。在对侧视神经再生后注入视顶盖的WGA-HRP再次标记一小片神经节细胞，表明再生终末也具有视网膜拓扑性的程度。
