O'Neill S J, Lesperance E, Klass D J
Am Rev Respir Dis. 1984 Dec;130(6):1177-9. doi: 10.1164/arrd.1984.130.6.1177.
We tested the effect of a preparation of human lung lavage surfactant (SAM) on phagocytosis and intracellular killing of Staphylococcus aureus by human alveolar macrophages (AM). When staphylococci were incubated with 100 to 300 micrograms of SAM, AM phagocytosis but not intracellular killing efficiency was enhanced. The mean numbers of intracellular staphylococci/AM were 36.0 +/- 2.7 and 23.3 +/- 2.8 in the SAM and control groups, respectively (p less than 0.001). The AM intracellular killing efficiency was 40.9 +/- 3.3% and 39.5 +/- 3.9% in the SAM and control groups, respectively (p greater than 0.05). Our results suggest that SAM is an important nonimmune opsonin for bacterial phagocytosis by human AM.
我们测试了人肺灌洗表面活性剂（SAM）制剂对人肺泡巨噬细胞（AM）吞噬和细胞内杀灭金黄色葡萄球菌的影响。当葡萄球菌与100至300微克的SAM一起孵育时，AM的吞噬作用增强，但细胞内杀灭效率未增强。SAM组和对照组中细胞内葡萄球菌/AM的平均数量分别为36.0±2.7和23.3±2.8（p<0.001）。SAM组和对照组中AM的细胞内杀灭效率分别为40.9±3.3%和39.5±3.9%（p>0.05）。我们的结果表明，SAM是人类AM吞噬细菌的重要非免疫调理素。
