Enhancement of amphotericin B selectivity by antibiotic incorporation into gel state vesicles. A circular dichroism and permeability study.

The permeability induced in cholesterol-or ergosterol-containing phospholipid vesicles by Amphotericin B incorporated into Dipalmitoyl Phosphatidylcholine vesicles has been studied in parallel with Circular Dichroism spectroscopy measurements. In our conditions, Amphotericin B is 5 to 10 times more selective to ergosterol- than to cholesterol-containing vesicles. Such a large difference is not observed when Amphotericin B is directly added to the vesicles suspension as its solution in organic solvent.