4-羟基壬烯醛在细胞损伤中的作用机制。

The mechanism of action of 4-hydroxynonenal in cell injury.

作者信息

White J S, Rees K R

出版信息

Chem Biol Interact. 1984 Dec;52(2):233-41. doi: 10.1016/0009-2797(84)90076-0.

Abstract

The effect of the C9 ketoaldehyde, 4-hydroxynonenal (HNE), a cytotoxic product of lipid peroxidation, on DNA, RNA and protein synthesis has been investigated in cells in culture. Macromolecular synthesis is powerfully inhibited by this agent which readily enters the lipid-rich membranes and is considerably more toxic than the polar ketoaldehyde, methyl glyoxal (MG). The entry of HNE into membranes lowers their glutathione GSH content. This is associated with an increased lipid peroxidation measured in vitro which is blocked by added GSH or alpha-tocopherol. It is proposed that this latter sequence of events is the underlying cause of the cytopathic effect of HNE in cells in culture.

摘要

脂质过氧化的细胞毒性产物C9酮醛4-羟基壬烯醛（HNE）对培养细胞中DNA、RNA和蛋白质合成的影响已得到研究。这种试剂能有效抑制大分子合成，它很容易进入富含脂质的膜，且毒性比极性酮醛甲基乙二醛（MG）大得多。HNE进入膜会降低其谷胱甘肽（GSH）含量。这与体外测量的脂质过氧化增加有关，而添加GSH或α-生育酚可阻断这种增加。有人提出，后一系列事件是HNE在培养细胞中产生细胞病变效应的根本原因。