Honda K, Komoda Y, Nishida S, Nagasaki H, Higashi A, Uchizono K, Inoué S
Neurosci Res. 1984 Aug;1(4):243-52. doi: 10.1016/s0168-0102(84)80003-6.
A 10-h intraventricular infusion of 10 pmol of uridine from 19.00 to 05.00 h resulted in significant increases in sleep in otherwise saline-infused male rats (n = 8) during the environmental dark period (20.00-08.00 h). Increments of slow wave sleep (SWS) and paradoxical sleep (PS) were 21.0% and 68.1%, respectively, of the baseline value. This was due to increases in the frequencies of both SWS and PS episodes but not to their durations. Similar increases occurred the first recovery night under saline infusion, but sleep amounts returned to the baseline levels the second night. Brain temperature was not affected by uridine administration. A small dose of uridine (1 pmol/10 h) exerted no effect (n = 6) while larger doses (100 and 1000 pmol/10 h, each n = 5) resulted in slight but insignificant increases in SWS and PS. The 1000-pmol uridine administration seemed to be non-physiological since it brought about irregularities in locomotor activity and sleep-waking rhythms. Thus, authentic uridine exhibited the same sleep-enhancing effects as a naturally occurring active component of sleep-promoting substance, which was recently identified with uridine.
在环境黑暗期（20:00 - 08:00），对原本输注生理盐水的雄性大鼠（n = 8）从19:00至05:00进行10小时的脑室内输注10皮摩尔尿苷，结果睡眠显著增加。慢波睡眠（SWS）和异相睡眠（PS）的增量分别为基线值的21.0%和68.1%。这是由于SWS和PS发作频率增加，而非发作持续时间增加所致。在输注生理盐水后的第一个恢复夜晚也出现了类似的增加，但第二个夜晚睡眠量恢复到了基线水平。尿苷给药未影响脑温。小剂量尿苷（1皮摩尔/10小时）无作用（n = 6），而较大剂量（100和1000皮摩尔/10小时，每组n = 5）导致SWS和PS略有增加但不显著。1000皮摩尔尿苷给药似乎是非生理性的，因为它导致了运动活动和睡眠 - 觉醒节律的不规则。因此，天然尿苷表现出与睡眠促进物质的天然活性成分相同的促睡眠作用，该活性成分最近被鉴定为尿苷。
