Jobe A H, Ikegami M, Jacobs H C, Jones S J
Am Rev Respir Dis. 1983 Jun;127(6):751-5. doi: 10.1164/arrd.1983.127.6.751.
To quantify the relationship between surfactant pool size and severity of respiratory disease, 21 lambs were delivered at 134 to 136 days gestational age and ventilated by varying only peak inspiratory pressure to maintain tidal volume at 6.2 +/- 0.3 ml/kg (mean +/- SE) and thus to control PCO2. Compliance measurements were used to quantify the severity of lung disease. After alveolar wash, surfactant phosphatidylcholine, saturated phosphatidylcholine, and minimal surface tensions were estimated. Compliance correlated linearly with saturated phosphatidylcholine pool size (r = 0.755, p less than 0.001). The mean minimal surface tension of the alveolar washes was 17.4 +/- 1.7 dynes/cm, and alveolar washes from lambs with more compliant lungs had lower minimal surface tensions than did washes from lambs with poorly compliant lungs (p less than 0.001). Lung tissue of all lambs contained similar amounts of saturated and total phosphatidylcholine, and in vitro rates of incorporation of labeled choline and palmitate into phosphatidylcholine in lung slices were similar, independent of severity of lung disease. The pool size of surfactant within the alveoli is an important determinant of lung disease in premature lambs; however, surface tension, tissue maturity, and other factors may contribute to the severity of the disease.
为了量化表面活性剂池大小与呼吸系统疾病严重程度之间的关系，21只胎羊在妊娠134至136天时出生，并仅通过改变吸气峰压进行通气，以维持潮气量在6.2±0.3 ml/kg（平均值±标准误），从而控制二氧化碳分压。通过测量顺应性来量化肺部疾病的严重程度。在进行肺泡灌洗后，对表面活性剂磷脂酰胆碱、饱和磷脂酰胆碱和最小表面张力进行了估算。顺应性与饱和磷脂酰胆碱池大小呈线性相关（r = 0.755，p < 0.001）。肺泡灌洗的平均最小表面张力为17.4±1.7达因/厘米，肺顺应性较好的胎羊的肺泡灌洗最小表面张力低于肺顺应性较差的胎羊（p < 0.001）。所有胎羊的肺组织中饱和磷脂酰胆碱和总磷脂酰胆碱的含量相似，且肺切片中标记胆碱和棕榈酸掺入磷脂酰胆碱的体外速率也相似，与肺部疾病的严重程度无关。肺泡内表面活性剂的池大小是早产胎羊肺部疾病的一个重要决定因素；然而，表面张力、组织成熟度和其他因素可能也会影响疾病的严重程度。
