Enzymatic synthesis of [6-14C]orotidine 5'-monophosphate and its use in the assay of orotate phosphoribosyltransferase and orotidylate decarboxylase.
作者信息
Rawls J M
出版信息
Anal Biochem. 1978 May;86(1):107-17. doi: 10.1016/0003-2697(78)90323-8.
PMID:655372
Abstract
摘要
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Enzymatic synthesis of [6-14C]orotidine 5'-monophosphate and its use in the assay of orotate phosphoribosyltransferase and orotidylate decarboxylase.
Anal Biochem. 1978 May;86(1):107-17. doi: 10.1016/0003-2697(78)90323-8.
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Orotate phosphoribosyltransferase: orotidylate decarboxylase (Serratia).乳清酸磷酸核糖基转移酶：乳清酸核苷酸脱羧酶（粘质沙雷氏菌）
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Purification of orotate phosphoribosyltransferase and orotidylate decarboxylase by affinity chromatography on Sepharose dye derivatives.通过琼脂糖染料衍生物亲和层析法纯化乳清酸磷酸核糖基转移酶和乳清酸核苷酸脱羧酶。
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Rapid conversion of newly-synthesized orotate to uridine-5-monophosphate by rat liver cytosolic enzymes.大鼠肝细胞溶质酶将新合成的乳清酸盐快速转化为5-磷酸尿苷。
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