Navarro A, Grisolía S
FEBS Lett. 1984 Feb 27;167(2):259-62. doi: 10.1016/0014-5793(84)80138-6.
Ornithine transcarbamylase (OTC) is very stable in rat liver homogenates, or mitochondria. However, pure OTC from rat or beef liver is inactivated by broken lysosomes. ATP and a number of purine nucleotides, particularly epsilon-ATP, stimulate the inactivation of OTC by broken lysosomes. Inactivation of OTC by trypsin and elastase is also stimulated by epsilon-ATP. Carbamoyl phosphate protects OTC against proteolytic attack by elastase or by broken lysosomes. Thus, environmental factors, e.g., ATP and carbamoyl phosphate, appear to influence OTC stability to proteases.
鸟氨酸转氨甲酰酶（OTC）在大鼠肝脏匀浆或线粒体中非常稳定。然而，来自大鼠或牛肝脏的纯OTC会被破碎的溶酶体灭活。ATP和许多嘌呤核苷酸，特别是ε-ATP，会刺激破碎的溶酶体对OTC的灭活作用。ε-ATP也会刺激胰蛋白酶和弹性蛋白酶对OTC的灭活。氨甲酰磷酸可保护OTC免受弹性蛋白酶或破碎溶酶体的蛋白水解攻击。因此，环境因素，如ATP和氨甲酰磷酸，似乎会影响OTC对蛋白酶的稳定性。
