Zimmermann U, Vienken J, Pilwat G, Arnold W M
Ciba Found Symp. 1984;103:60-85. doi: 10.1002/9780470720844.ch5.
Exposure of cells or liposomes to a brief pulse of a strong electrical field can result in a reversible breakdown of the outer membrane. Such breakdown results in an increase in permeability of the plasmalemma, which however re-seals after a short incubation (i.e. the original impermeability is restored). Two or more cells in contact can be made to fuse by this process, provided that the contact is close enough and that the pulse of the electrical field is short enough not to damage the cells. Methods of achieving this contact by electrical and magnetic fields are described. The magnetic method does not demand the use of the low conductivity media used earlier. Other possible modifications of this flexible technique are also described, and used to show how the technique can be modified in future, and how it may be applied to the fields of membrane research, medicine and plant breeding.
将细胞或脂质体暴露于强电场的短暂脉冲下，可能会导致外膜可逆性破裂。这种破裂会导致质膜通透性增加，但在短暂孵育后会重新封闭（即恢复原来的不透性）。只要接触足够紧密且电场脉冲足够短以至于不会损伤细胞，通过这个过程可以使两个或更多相互接触的细胞融合。文中描述了通过电场和磁场实现这种接触的方法。磁性方法不需要使用先前使用的低电导率介质。还描述了这种灵活技术的其他可能改进，并用于展示该技术未来如何改进以及如何应用于膜研究、医学和植物育种领域。
