电脉冲诱导原生质体融合用于电泳。

Electrically induced protoplast fusion using pulse electric fields for dielectrophoresis.

机构信息

Bulgarian Academy of Sciences, Institute of Plant Physiology, Acad. G. Bonchev str., bl 21, 1113 Sofia, Bulgaria.

出版信息

Plant Physiol. 1992 Dec;100(4):2008-12. doi: 10.1104/pp.100.4.2008.

DOI:10.1104/pp.100.4.2008

PMID:16653231

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1075898/

Abstract

The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formation of protoplast chains in the presence of CaCl(2) up to 5 mm, and protoplast fusion in the presence of 3 mm CaCl(2) were found when the pulse field was applied. The present results suggest the possibility of electrically induced protoplast fusion at cation concentrations that prevent fusion when sine-wave fields are applied.

摘要

电场诱导分离的豌豆（Pisum sativum cv Ran 1）叶肉原生质体悬浮液中原生质体链的形成。研究了正弦波场（2 V，峰峰值；500-0.1 kHz）诱导的链形成与交流脉冲场（1 V，幅度；0.1-0.4 kHz）诱导的链形成的区别。结果发现，在施加脉冲场时，增加了电介质成对原生质体的数量，在存在 CaCl2 的情况下形成原生质体链，浓度高达 5 mM，并且在存在 3 mM CaCl2 的情况下发生原生质体融合。当正弦波场施加时，阳离子浓度可防止融合，本结果提示电诱导原生质体融合的可能性。