Bulgarian Academy of Sciences, Institute of Plant Physiology, Acad. G. Bonchev str., bl 21, 1113 Sofia, Bulgaria.
Plant Physiol. 1992 Dec;100(4):2008-12. doi: 10.1104/pp.100.4.2008.
The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formation of protoplast chains in the presence of CaCl(2) up to 5 mm, and protoplast fusion in the presence of 3 mm CaCl(2) were found when the pulse field was applied. The present results suggest the possibility of electrically induced protoplast fusion at cation concentrations that prevent fusion when sine-wave fields are applied.
电场诱导分离的豌豆（Pisum sativum cv Ran 1）叶肉原生质体悬浮液中原生质体链的形成。研究了正弦波场（2 V，峰峰值；500-0.1 kHz）诱导的链形成与交流脉冲场（1 V，幅度；0.1-0.4 kHz）诱导的链形成的区别。结果发现，在施加脉冲场时，增加了电介质成对原生质体的数量，在存在 CaCl2 的情况下形成原生质体链，浓度高达 5 mM，并且在存在 3 mM CaCl2 的情况下发生原生质体融合。当正弦波场施加时，阳离子浓度可防止融合，本结果提示电诱导原生质体融合的可能性。
