Sharp S, Dingermann T, Schaack J, Sharp J A, Burke D J, DeRobertis E M, Söll D
Nucleic Acids Res. 1983 Dec 20;11(24):8677-90. doi: 10.1093/nar/11.24.8677.
The intragenic control regions of a eukaryotic tRNA gene have been examined by transcribing mutant forms of a Drosophila tRNAArg gene either by injection into the nucleus of Xenopus oocytes or in extracts prepared from isolated oocyte nuclei. These experiments demonstrate that the selection of the transcription initiation site is a complex mechanism that involves the T-control region, the D-control region, and sequences 5' adjacent to the D-control region. In this study either "half" of the Drosophila tRNAArg gene promoted transcription in Xenopus oocytes. This finding supports a recent model for eukaryotic tRNA gene transcription (Dingermann et al., 1983, J. Biol. Chem. 258, 10395-10402) that proposes transcription initiation is dependent on the ability of specific DNA sequences to sequester two RNA polymerase III transcription factors.
通过将果蝇tRNAArg基因的突变形式注射到非洲爪蟾卵母细胞核中，或在从分离的卵母细胞核制备的提取物中进行转录，对真核生物tRNA基因的基因内控制区域进行了研究。这些实验表明，转录起始位点的选择是一个复杂的机制，涉及T控制区域、D控制区域以及与D控制区域相邻的5'序列。在本研究中，果蝇tRNAArg基因的任一“半体”都能在非洲爪蟾卵母细胞中促进转录。这一发现支持了最近关于真核生物tRNA基因转录的模型（丁格曼等人，1983年，《生物化学杂志》258卷，10395 - 10402页），该模型提出转录起始取决于特定DNA序列隔离两种RNA聚合酶III转录因子的能力。
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