来自医用水蛭 Hirudo medicinalis 的水蛭素对人白细胞弹性蛋白酶的抑制动力学。
Kinetics of the inhibition of human leucocyte elastase by eglin from the leech Hirudo medicinalis.
作者信息
Baici A, Seemüller U
出版信息
Biochem J. 1984 Mar 15;218(3):829-33. doi: 10.1042/bj2180829.
Abstract
The rate constants for the inhibition of human leucocyte elastase by eglin from the leech Hirudo medicinalis were determined by using a pre-steady-state kinetic approach. kon and koff for complex-formation and dissociation were 1 X 10(6)M-1 X S-1 and 8 X 10(-4)S-1 respectively. Ki was calculated as the ratio koff/kon = 8 X 10(-10)M, the binding of eglin to elastase was reversible and the inhibition mechanism was of the fully competitive type. The mechanistic properties of the system and the biological significance of the rate constants are discussed.
摘要
采用预稳态动力学方法测定了来自医用水蛭 Hirudo medicinalis 的水蛭素对人白细胞弹性蛋白酶的抑制速率常数。复合物形成和解离的 kon 和 koff 分别为 1×10(6)M-1×S-1 和 8×10(-4)S-1。计算得出 Ki 为 koff/kon 的比值，即 8×10(-10)M，水蛭素与弹性蛋白酶的结合是可逆的，抑制机制为完全竞争型。讨论了该系统的机制特性和速率常数的生物学意义。
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