Tokumura A, Harada K, Yoshioka Y, Tsukatani H, Handa Y
Biomed Mass Spectrom. 1984 Apr;11(4):167-71. doi: 10.1002/bms.1200110406.
Lysophosphatidic acids having different fatty acyl moieties were directly analysed by gas chromatography/mass spectrometry after silylation. Trimethylsilyl derivatives of various LPAs escaped thermal degradation, and electron impact and chemical ionization of these derivatives yielded many ions which were useful for the structural determination. It was found by this new method that five species of lysophosphatidic acids were formed in rat plasma during incubation at 37 degrees C.
具有不同脂肪酰基部分的溶血磷脂酸在硅烷化后通过气相色谱/质谱法直接进行分析。各种溶血磷脂酸的三甲基硅烷基衍生物避免了热降解，并且这些衍生物的电子轰击和化学电离产生了许多有助于结构测定的离子。通过这种新方法发现，在37℃孵育期间大鼠血浆中形成了五种溶血磷脂酸。
