Tarannum Nazia, Kumar Deepak, Agrawal Ranu
Department of Chemistry, Chaudhary Charan Singh University, Meerut, India.
Department of Applied Science, Sir Chhotu Ram Institute of Engineering and Technology, Chaudhary Charan Singh University, Meerut, India.
J Cancer Prev. 2023 Jun 30;28(2):31-39. doi: 10.15430/JCP.2023.28.2.31.
Herein, an instrument free facile acid-base titrimetric methodology is reported for lysophosphatidic acid (LPA) measurement in serum and plasma samples for ovarian cancer detection. The concept is based on the titrimetric method in which alkaline solution was titrated with free fatty acid. Free fatty acid is generated due to action of the lysophospholipase to LPA. A phospholipid derivative known as LPA can function as a signaling molecule. A glycerol backbone serves as the foundation for phosphatidic acid, which also has bonds to an unsaturated fatty acid at carbon-1, a hydroxyl group at carbon-2, and a phosphate molecule at carbon-3. Free fatty acid and glycerol-3-phosphate are formed when LPA reacts with lysophospholipase. The formation of free fatty acid depends on the concentration of LPA. The standard graph of known concentrations of LPA, LPA spiked serum and LPA spiked plasma was plotted. The concentration of LPA in unknown serum and plasma were calculated from the standard graph. The limit of detection of LPA in spiked serum and plasma samples via titrimetric assay was calculated as 0.156 μmol/L. A patient's chance of survival may be outweighed by an early diagnosis of ovarian cancer.
在此，我们报道了一种无需仪器的简便酸碱滴定法，用于检测血清和血浆样本中的溶血磷脂酸（LPA）以诊断卵巢癌。该方法基于酸碱滴定法，即用游离脂肪酸滴定碱性溶液。溶血磷脂酶作用于LPA会产生游离脂肪酸。一种名为LPA的磷脂衍生物可作为信号分子。甘油骨架是磷脂酸的基础，磷脂酸在碳-1处与不饱和脂肪酸相连，在碳-2处有一个羟基，在碳-3处有一个磷酸分子。LPA与溶血磷脂酶反应时会形成游离脂肪酸和3-磷酸甘油。游离脂肪酸的形成取决于LPA的浓度。绘制了已知浓度的LPA、添加LPA的血清和添加LPA的血浆的标准曲线。从未知血清和血浆中LPA的标准曲线计算其浓度。通过滴定法测定添加LPA的血清和血浆样本中LPA的检测限为0.156μmol/L。卵巢癌的早期诊断可能会提高患者的生存几率。
