红细胞裂解物对电位敏感碳菁染料光谱的差异效应。

Differential effects of erythrocyte lysates on spectra of potential-sensing carbocyanine dyes.

作者信息

Guillet E, Kimmich G

出版信息

Biochim Biophys Acta. 1978 May 18;509(2):385-8. doi: 10.1016/0005-2736(78)90056-1.

Abstract

A recent report by Hladky, S.B. and Rink, T.J. ((1976) J. Physiol. 263, 287--319) demonstrates the binding of a thiocarbocyanine dye (di-S-C3 (5)) to an intracellular constituent from human erythrocytes. Evidence presented below shows that the binding of carbocyanine dyes depends on the particular type of dye used and on the species of erythrocyte from which cellular constituents are prepared. It is recommended that dyes which show minimal binding to intracellular components be used for investigations where carbocyanine dyes are employed as probes of membrane potential.

摘要

Hladky, S.B.和Rink, T.J.最近的一份报告（(1976)《生理学杂志》263卷，287 - 319页）证明了一种硫代碳氰化物染料（二-S-C3(5)）与人红细胞中的一种细胞内成分结合。下面给出的证据表明，碳氰化物染料的结合取决于所使用染料的特定类型以及制备细胞成分所取自的红细胞种类。建议在将碳氰化物染料用作膜电位探针的研究中，使用与细胞内成分结合最少的染料。