Guillet E G, Kimmich G A
J Membr Biol. 1981 Mar 15;59(1):1-11. doi: 10.1007/BF01870815.
The experiments presented below compare the interaction of diO-C3-(5) and diS-C3-(5) with erythrocytes, erythrocyte ghosts and phospholipid vesicles derived from erythrocyte membranes. The results confirm earlier reports of diS-C3-(5) dimerization in the presence of hemoglobin and of dye aggregate formation in erythrocyte suspensions. DiO-C3-(5), on the other hand, binds to vesicles and ghosts freed of hemoglobin in a potential-dependent manner but without forming dye aggregates. The two dyes bind to the different preparations in similar proportions, but diS-C3-(5) is bound in amounts 3-40 times greater depending on the degree of polarization. The results show that mechanism other than binding to hemoglobin must occur in order to explain the potential-dependent binding of both dyes to ghosts and vesicles. A primary interaction must exist between the dye molecule and the lipid bilayer in a biological membrane, and this would be expected to occur in the presence of hemoglobin or other cytosolic components. DiO-C3-(5) is a better dye to use than diS-C3-(5) for mechanistic studies, in order to avoid problems associated with formation of complex aggregates of the latter dye, especially in hyperpolarized membrane suspensions.
以下实验比较了二辛基 - C3 - (5) 和二硫代 - C3 - (5) 与红细胞、红细胞血影以及源自红细胞膜的磷脂囊泡的相互作用。结果证实了先前关于二硫代 - C3 - (5) 在血红蛋白存在下二聚化以及在红细胞悬浮液中形成染料聚集体的报道。另一方面，二辛基 - C3 - (5) 以电位依赖的方式与去除血红蛋白的囊泡和血影结合，但不形成染料聚集体。两种染料以相似的比例与不同制剂结合，但根据极化程度，二硫代 - C3 - (5) 的结合量要大 3 - 40 倍。结果表明，为了解释两种染料与血影和囊泡的电位依赖结合，必须存在除与血红蛋白结合之外的机制。染料分子与生物膜中的脂质双层之间必定存在主要相互作用，并且预计这种相互作用会在血红蛋白或其他胞质成分存在的情况下发生。为了避免与后一种染料形成复杂聚集体相关的问题，尤其是在超极化膜悬浮液中，在进行机制研究时，二辛基 - C3 - (5) 比二硫代 - C3 - (5) 更适合作为染料使用。
