Owen C S, Lindsay J G
Biophys J. 1983 May;42(2):145-50. doi: 10.1016/S0006-3495(83)84380-X.
In three model systems, particles the size of cells or smaller have been surface labeled with ferritin to make them slightly paramagnetic, by virtue of the iron in the ferritin. In each case it was possible to show that labeled particles could be magnetically removed from a flowing suspension by the high-gradient magnetic separation (HGMS) technique. The first system of particles consisted of small (1 micron) carboxylate-modified latex spheres to which ferritin was covalently bound to create stable paramagnetic particles analogous to a ferritin-labeled subcellular membrane preparation. In the second system polyacrylamide beads that more closely approximated whole cells in size (5-50 microns) were labeled with immunoferritin. The third system was a biomembrane preparation: erythrocyte ghosts labeled with a ferritin-lectin conjugate. A field of 7 T (tesla) (70 kG) was used in each case, along with buffer flow rates through the HGMS column in the range 0.1-1.0 ml/min.
在三个模型系统中,细胞大小或更小的颗粒已通过用铁蛋白进行表面标记,借助铁蛋白中的铁使其具有轻微顺磁性。在每种情况下,都能够证明标记的颗粒可以通过高梯度磁分离(HGMS)技术从流动的悬浮液中磁性去除。第一个颗粒系统由小的(1微米)羧酸盐修饰的乳胶球组成,铁蛋白与乳胶球共价结合,以产生类似于铁蛋白标记的亚细胞膜制剂的稳定顺磁性颗粒。在第二个系统中,大小更接近完整细胞(5 - 50微米)的聚丙烯酰胺珠用免疫铁蛋白进行标记。第三个系统是一种生物膜制剂:用铁蛋白 - 凝集素缀合物标记的红细胞血影。每种情况下都使用了7特斯拉(70千高斯)的磁场,同时缓冲液通过HGMS柱的流速在0.1 - 1.0毫升/分钟范围内。