Galust'ian G E, Prianishnikov V A
Biull Eksp Biol Med. 1978 May;85(5):623-6.
Modification of the histochemical method for detection of GABA-transaminase activity is suggested. Optimal concentrations of the substrates and cofactors are chosen on the basis of kinetic study of the enzymatic reaction in the cryostat sections of the rat cerebellar cortex by the quantitative microspectrophotometric method. The method is intended for the quantitative histochemical analysis of GABA-transaminase activity in the brain sections.
建议对检测γ-氨基丁酸转氨酶活性的组织化学方法进行改进。通过定量显微分光光度法对大鼠小脑皮质低温切片中的酶促反应进行动力学研究,以此来选择底物和辅因子的最佳浓度。该方法旨在对脑切片中γ-氨基丁酸转氨酶活性进行定量组织化学分析。