Ullu E, Farace M G, Gambari R, Orsi P, Lunadei M, Fantoni A
Blood. 1978 Jul;52(1):171-80.
The kinetic relationship between the globin mRNA accumulation and the rate of synthesis of globin chains was studied during the terminal stages of differentiation in erythroid cells derived from the yolk sac of mouse fetuses. RNA derived from the whole cells and from different cell compartments were hybridized to DNA complementary to embryonic globin mRNA. The relative proportion of embryonic globin RNA molecules and their absolute number per cell were estimated on the 11th, 12th, and 13th days of mouse fetal development. During erythroid terminal differentiation globin mRNA became progressively predominant on polyribosomes along with the progressive specialization of cell functions. The number of embryonic globin RNA molecules per cell remained constant while yolk sac erythroid cells underwent two rounds of cell division. These data indicate that the transcription of globin genes is operative throughout the last stages of terminal differentiation and that there is no detectable storage of globin RNA sequences in these cells. The rates of accumulation of mRNA molecules and of globin synthesis both seem correlated to the length of the cell cycle.
在源自小鼠胎儿卵黄囊的红细胞终末分化阶段，研究了珠蛋白mRNA积累与珠蛋白链合成速率之间的动力学关系。从全细胞和不同细胞区室获得的RNA与胚胎珠蛋白mRNA的互补DNA杂交。在小鼠胎儿发育的第11、12和13天，估计了胚胎珠蛋白RNA分子的相对比例及其每个细胞的绝对数量。在红细胞终末分化过程中，随着细胞功能的逐步特化，珠蛋白mRNA在多核糖体上逐渐占主导地位。当卵黄囊红细胞经历两轮细胞分裂时，每个细胞中胚胎珠蛋白RNA分子的数量保持不变。这些数据表明，珠蛋白基因的转录在终末分化的最后阶段一直起作用，并且在这些细胞中没有可检测到的珠蛋白RNA序列储存。mRNA分子的积累速率和珠蛋白的合成速率似乎都与细胞周期的长度相关。
