小鼠胚胎γ-珠蛋白与成年β-珠蛋白信使核糖核酸之间的序列差异。

Sequence differences between mouse embryonic y- and adult beta-globin messenger ribonucleic acids.

作者信息

Soma G I, Obinata M, Ikawa Y

出版信息

Biochemistry. 1980 Aug 19;19(17):3967-73. doi: 10.1021/bi00558a012.

Abstract

Poly(A)-containing RNA was isolated from yolk sac cells of BDF1 hybrid mice on the 12th day of gestation. In vitro translation of the poly(A)-containing RNA by a messenger RNA (mRNA) dependent reticulocyte lysate system revealed that this RNA contained a high concentration of mRNAs from two embryonic globin chain subunits, y-1 and y-2. Cross hybridization of complementary DNA (cDNA) to the embryonic globin mRNAs with adult reticulocyte globin mRNAs and with cloned cDNAs for adult alpha- and beta-globin chain mRNAs showed that the sequence of embryonic globin y-chain mRNA differed from that of adult beta-chain mRNA.

摘要

在妊娠第12天从BDF1杂交小鼠的卵黄囊细胞中分离出含poly(A)的RNA。通过依赖信使核糖核酸（mRNA）的网织红细胞裂解物系统对含poly(A)的RNA进行体外翻译，结果显示该RNA含有来自两个胚胎珠蛋白链亚基γ-1和γ-2的高浓度mRNA。将互补DNA（cDNA）与胚胎珠蛋白mRNA、成年网织红细胞珠蛋白mRNA以及成年α-和β-珠蛋白链mRNA的克隆cDNA进行交叉杂交，结果表明胚胎珠蛋白γ链mRNA的序列与成年β链mRNA的序列不同。