Wiesel F A, Fuxe K, Hökfelt T, Agnati L F
Brain Res. 1978 Jun 16;148(2):399-411. doi: 10.1016/0006-8993(78)90728-x.
The effects of 17 beta-estradiol benzoate (EB), ethynodioldiacetate and ovine prolactin on dopamine (DA) turnover have been studied. As an index for a change in turnover, differences in DA depletion following tyrosine hydroxylase inhibition with alpha-methyl-tyrosine methylester were observed. DA was measured by means of mass fragmentographical analysis in the rat median eminence, the olfactory tubercle and the striatum. The actions of ethynodioldiacetate and ovine prolactin on DA turnover in various subregions of the rat median eminence were analysed by quantitative microfluorimetry. Repeated injections of EB to ovariectomized rats resulted in a significant increase of DA turnover in the median eminence. Administration of ethynodioldiacetate to ovariectomized rats almost significantly increased DA turnover in the olfactory tubercle. In the median eminence DA turnover was significantly increased only in the lateral palisade zone. In male hypophysectomized rats ovine prolactin increased DA turnover in both the lateral and the medial palisade zone of the median eminence. The results support the involvement of DA neurons in the control of prolactin and luteinizing hormone secretion. It is suggested that the tubero-infundibular DA neurons are involved in mediating the central inhibitory feedback actions of prolactin and gonadal steroids on prolactin and LH secretion, respectively.
研究了苯甲酸雌二醇（EB）、炔诺酮二乙酸酯和羊催乳素对多巴胺（DA）代谢的影响。作为代谢变化的指标，观察了用α-甲基酪氨酸甲酯抑制酪氨酸羟化酶后DA耗竭的差异。通过质量碎片分析在大鼠正中隆起、嗅结节和纹状体中测量DA。通过定量显微荧光法分析了炔诺酮二乙酸酯和羊催乳素对大鼠正中隆起各亚区DA代谢的作用。对去卵巢大鼠重复注射EB导致正中隆起中DA代谢显著增加。给去卵巢大鼠注射炔诺酮二乙酸酯几乎显著增加了嗅结节中的DA代谢。在正中隆起中，仅外侧栅栏区的DA代谢显著增加。在雄性垂体切除大鼠中，羊催乳素增加了正中隆起外侧和内侧栅栏区的DA代谢。结果支持DA神经元参与催乳素和促黄体生成素分泌的控制。提示结节-漏斗部DA神经元分别参与介导催乳素和性腺类固醇对催乳素和LH分泌的中枢抑制性反馈作用。
