Rubin K, Borg T K, Holmdahl R, Klareskog L, Obrink B
Ciba Found Symp. 1984;108:93-116. doi: 10.1002/9780470720899.ch7.
Isolated stationary cells recognize and adhere to immobilized extracellular matrix (ECM) components. These processes are mediated by specific receptor-ligand types of interaction. The formation of cell-ECM contacts influences subsequent cellular behaviour, such as the promotion of cell survival, epithelial cell polarization, and cell differentiation. The binding reaction between cells and collagen is discussed in this paper in terms of findings from studies with two cell systems, adult rat hepatocytes and rat cardiac myocytes. Isolated adult rat hepatocytes adhere to or bind laminin, fibronectin, heparan sulphate and collagen. We have characterized the interaction of hepatocytes with collagen and have isolated a glycoprotein fraction from rat liver membranes that contains collagen-binding components. The ability of rat cardiac myocytes to recognize ECM components depends on the state of cell maturation. Myocytes isolated from neonatal rats adhere to the interstitial collagens (types I-III), to collagen types IV and V, to fibronectin and to laminin. In contrast, myocytes isolated from adult rats bind effectively only to collagen type IV and laminin and bind much less effectively to fibronectin. Furthermore, antibodies raised against neonatal myocyte membranes inhibit the adhesion of both neonatal myocytes and hepatocytes to interstitial collagens, but antibodies raised against adult myocyte membranes lack these effects. These observations indicate that similar collagen-binding molecules are present on such diverse cells as hepatocytes and neonatal myocytes and that these components might be lost during cell maturation.
分离的静止细胞能够识别并黏附于固定化的细胞外基质（ECM）成分。这些过程由特定的受体-配体相互作用类型介导。细胞与ECM接触的形成会影响后续的细胞行为，如促进细胞存活、上皮细胞极化和细胞分化。本文根据对成年大鼠肝细胞和大鼠心肌细胞这两种细胞系统的研究结果，讨论了细胞与胶原蛋白之间的结合反应。分离的成年大鼠肝细胞能黏附或结合层粘连蛋白、纤连蛋白、硫酸乙酰肝素和胶原蛋白。我们已经对肝细胞与胶原蛋白的相互作用进行了表征，并从大鼠肝细胞膜中分离出了一个含有胶原蛋白结合成分的糖蛋白组分。大鼠心肌细胞识别ECM成分的能力取决于细胞成熟状态。从新生大鼠分离的心肌细胞能黏附于间质胶原（I-III型）、IV型和V型胶原、纤连蛋白和层粘连蛋白。相比之下，从成年大鼠分离的心肌细胞仅能有效结合IV型胶原和层粘连蛋白，而与纤连蛋白的结合效率则低得多。此外，针对新生心肌细胞膜产生的抗体可抑制新生心肌细胞和肝细胞与间质胶原的黏附，但针对成年心肌细胞膜产生的抗体则没有这些作用。这些观察结果表明，在肝细胞和新生心肌细胞等不同细胞上存在相似的胶原蛋白结合分子，并且这些成分可能在细胞成熟过程中丢失。
